Rebolledo E, Andrés M D, Taboada M C, Fernández-Otero M P
Departamento de Fisiología, Facultad de Biología, Santiago de Compostela.
Rev Esp Fisiol. 1988 Dec;44(4):395-9.
The effect of methionine dietary deficiency on food intake, weight gain, liver and kidney weight, feed conversion rate, protein efficiency ratio, maltase and leucineaminopeptidase (LAPase) activities of the intestinal mucosa as well as renal LAPase activity was studied. Three groups of female Wistar rats, weighing between 40-60 g, were fed for 25 days on either Diet A (casein supplemented with 0.6% DL-methionine), Diet B (amino acid mixture simulating casein also supplemented with 0.6% methionine) or Diet C (amino acid mixture with 0.67% methionine deficiency with respect to Diet A). The results show no significant differences in either growth or enzymatic activity between the rats fed on Diet A and those on Diet B. The animals fed on Diet C show an increase in intestinal (P less than 0.01, vs Diet B) and renal (P less than 0.005, vs Diet A) LAPase activity, although intestinal maltase activity remained unchanged. Food intake, weight gain, organ weight and nutritional parameters obtained in rats fed on Diet C showed no statistically significant changes, with the exception of kidney weight which decreased (P less than 0.005) when compared to those fed on Diet B.
研究了日粮中蛋氨酸缺乏对食物摄入量、体重增加、肝脏和肾脏重量、饲料转化率、蛋白质效率比、肠黏膜麦芽糖酶和亮氨酸氨肽酶（LAPase）活性以及肾脏LAPase活性的影响。将三组体重在40 - 60克之间的雌性Wistar大鼠，分别用日粮A（添加0.6% DL - 蛋氨酸的酪蛋白）、日粮B（模拟酪蛋白的氨基酸混合物，也添加0.6%蛋氨酸）或日粮C（相对于日粮A，蛋氨酸缺乏0.67%的氨基酸混合物）喂养25天。结果显示，喂养日粮A的大鼠和喂养日粮B的大鼠在生长或酶活性方面均无显著差异。喂养日粮C的动物，其肠LAPase活性（与日粮B相比，P < 0.01）和肾LAPase活性（与日粮A相比，P < 0.005）有所增加，尽管肠麦芽糖酶活性保持不变。与喂养日粮B的大鼠相比，喂养日粮C的大鼠在食物摄入量、体重增加、器官重量和营养参数方面均无统计学上的显著变化，但肾脏重量有所下降（P < 0.005）。
