马铃薯品种对属于马铃薯Y病毒四个株系的五个分离物的反应

Response of Potato Cultivars to Five Isolates Belonging to Four Strains of Potato virus Y.

作者信息

Nie Bihua, Singh Mathuresh, Murphy Agnes, Sullivan Andrew, Xie Conghua, Nie Xianzhou

机构信息

National Center for Vegetable Improvement (Central China), MOE Key Laboratory of Horticultural Plant Biology, Hubei Provincial Research Center of Potato Engineering and Technology, Huazhong Agricultural University, Wuhan, 430070, China, and Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, 850 Lincoln Road, Fredericton, New Brunswick, E3B 4Z7, Canada.

Agricultural Certification Services, 1030 Lincoln Road, Fredericton, New Brunswick, E3B 8B7, Canada.

出版信息

Plant Dis. 2012 Oct;96(10):1422-1429. doi: 10.1094/PDIS-01-12-0018-RE.

DOI:10.1094/PDIS-01-12-0018-RE

PMID:30727313

Abstract

The responses of 14 potato cultivars to five Potato virus Y (PVY) isolates belonging to four strains (ordinary [PVY], tobacco veinal necrosis [PVY], N:O group [PVY], and nonrecombinant potato tuber necrotic [PVY]) were studied in primary and secondary infections. For the primary infection experiments, foliage symptoms were monitored daily after mechanical inoculation with a PVY isolate until harvest; and, for the secondary infection experiments, foliage symptoms were monitored regularly from plant emergence until harvest. Tuber symptoms (namely, tuber necrotic ringspots) were checked at harvest and monthly postharvest for up to 4 months. In both infections, symptoms varied significantly depending on potato cultivar and virus strain or isolate. In primary infections, local lesions occurred on inoculated leaves of 'AC Chaleur', 'Eramosa', 'Goldrush', 'Jemseg', 'Katahdin', 'Ranger Russet', and 'Yukon Gold' after inoculation with PVY isolates, followed by systemic necrosis on latterly emerged uninoculated leaves. In contrast, plants of 'CalWhite', 'La Rouge', 'Red LaSoda', 'Russet Burbank', 'Russet Norkotah', and 'Superior' did not exhibit any visible symptoms on inoculated leaves but developed mild to severe mosaic on latterly emerged leaves after infection with PVY isolates. In all cultivars, near-symptomless to mild mosaic was induced by PVY and mild to severe mosaic by PVY. PVY induced mild to severe mosaic in plants of all cultivars except AC Chaleur, 'Cherokee', and Yukon Gold, which developed visible systemic necrosis. Necrotic ringspots were observed in tubers of PVY-infected plants of AC Chaleur, Cherokee, and Yukon Gold. The tuber symptoms were also incited by PVY-Jg on Cherokee. In secondary infections, the symptoms were generally more severe than primary infections even though the symptom types did not alter. As in the greenhouse, a clear symptom severity pattern (PVY-FL > PVY-RB > PVY-Sl > PVY-Mb58 > PVY-Jg) was observed in AC Chaleur, Cherokee, Eramosa, Goldrush, Jemseg, Katahdin, Ranger Russet, and Yukon Gold in the field.

摘要

研究了14个马铃薯品种对属于4个株系（普通株系[PVY]、烟草脉坏死株系[PVY]、N:O组株系[PVY]和非重组马铃薯块茎坏死株系[PVY]）的5种马铃薯Y病毒（PVY）分离株在初次感染和二次感染中的反应。在初次感染实验中，用PVY分离株机械接种后，每天监测叶片症状直至收获；在二次感染实验中，从植株出土到收获定期监测叶片症状。在收获时及收获后每月检查块茎症状（即块茎坏死环斑），持续4个月。在这两种感染中，症状因马铃薯品种和病毒株系或分离株的不同而有显著差异。在初次感染中，用PVY分离株接种后，“AC Chaleur”“Eramosa”“Goldrush”“Jemseg”“Katahdin”“Ranger Russet”和“Yukon Gold”的接种叶片上出现局部病斑，随后在后期长出的未接种叶片上出现系统坏死。相比之下，“CalWhite”“La Rouge”“Red LaSoda”“Russet Burbank”“Russet Norkotah”和“Superior”的植株在接种叶片上未表现出任何可见症状，但在感染PVY分离株后，后期长出的叶片上出现轻度至重度花叶病。在所有品种中，PVY诱导出近无症状至轻度花叶病，PVY诱导出轻度至重度花叶病。PVY在除AC Chaleur、“Cherokee”和Yukon Gold以外的所有品种植株中诱导出轻度至重度花叶病，这三个品种出现了可见的系统坏死。在AC Chaleur、Cherokee和Yukon Gold的PVY感染植株的块茎中观察到坏死环斑。PVY-Jg也在Cherokee上引发了块茎症状。在二次感染中，尽管症状类型没有改变，但症状通常比初次感染更严重。与温室中一样，在田间的AC Chaleur、Cherokee、Eramosa、Goldrush、Jemseg、Katahdin、Ranger Russet和Yukon Gold中观察到了明显的症状严重程度模式（PVY-FL > PVY-RB > PVY-Sl > PVY-Mb58 > PVY-Jg）。