Ledger S E, Deroles S C, Manson D G, Bradley J Marie, Given N K
Levin Research Centre, Crop and Food Research, Private Bag 4005, Levin, New Zealand Fax no.: 64-6-368-3578; E-mail:
Plant Cell Rep. 1997 Oct;16(12):853-858. doi: 10.1007/s002990050333.
Transformed plants from three cultivars of Eustoma grandiflorum (lisianthus) were produced by cocultivating young leaf pieces with Agrobacterium tumefaciens strain A722 containing the binary vectors pKIWI110 and pLN26. Both vectors contain the selectable marker gene for neomycin phosphotransferase II. pKIWI110 also contains the reporter gene for β-D-glucuronidase, and pLN26, the chalcone synthase antisense gene. Southern DNA analysis revealed that all the kanamycin-resistant transformants tested contained copies of the transgenes integrated in their genome. The two plants transformed with pKIWI110 show β-D-glucuronidase expression in their mature leaves and selected transformants passed on the kanamycin-resistant phenotype to the F1 generation.
通过将幼嫩叶片与含有二元载体pKIWI110和pLN26的根癌农杆菌菌株A722共培养，培育出了三种洋桔梗（草原龙胆）栽培品种的转化植株。这两种载体都含有新霉素磷酸转移酶II的选择标记基因。pKIWI110还含有β-D-葡萄糖醛酸酶的报告基因，而pLN26含有查尔酮合酶反义基因。Southern DNA分析表明，所有测试的卡那霉素抗性转化体在其基因组中都含有整合的转基因拷贝。用pKIWI110转化的两株植物在其成熟叶片中表现出β-D-葡萄糖醛酸酶表达，并且所选转化体将卡那霉素抗性表型传递给了F1代。
