Kim Y W, Youn Y, Noh E R, Kim J C
Department of Biotechnology, Forest Genetics Research Institute, P. O. Box 24, Suwon, Kyonggido 441-350, Republic of Korea, , , , , , KR.
Department of Biology, Kangwon National University, Chuncheon, Kangwondo 200-701, Republic of Korea, , , , , , KR.
Plant Cell Rep. 1997 Oct;16(12):869-873. doi: 10.1007/s002990050336.
Immature embryos of sawtooth oak (Quercus acutissima Carruth.) were obtained from five seed families and cultured on modified Murashige and Skoog nutrient medium containing 1 g/l L-glutamine and 5 mM proline and supplemented with 1.0 mg/l indole-3-butyric acid and 1.0 mg/l 6-benzylaminopurine. The frequency of somatic embryogenesis from immature embryos was a function of the collection date and seed family. The highest frequency of explants forming somatic embryos was obtained with seeds of family Chungnam 11, i.e. 7 weeks post-fertilization (90.9%) and 9 weeks post-fertilization (91.2%). No response was shown by families Chungnam 14, 15 and Jeonbook 29 (0%), at 10 weeks post fertilization. During germination, the highest frequency of epicotyl formation was obtained with Chungnam 11 (44.0%) or Chungnam 15 (43.5%), and the highest rate of radicle formation was shown by Chungnam 11 (26.1%). The most responsive seed family with respect to the formation of both epicotyl (43.5%) and radicle (26.1%) was Chungnam 11. Twenty plantlets were transplanted to a perlite:peatmoss:vermiculite (1:1:1) soil mixture, and 8 plants survived in the field.
从五个种子家系获取麻栎（Quercus acutissima Carruth.）未成熟胚，并在添加了1 g/L L-谷氨酰胺和5 mM脯氨酸的改良Murashige和Skoog营养培养基上培养，同时补充1.0 mg/L吲哚-3-丁酸和1.0 mg/L 6-苄基腺嘌呤。未成熟胚体细胞胚胎发生的频率是采集日期和种子家系的函数。形成体细胞胚的外植体最高频率是用忠南11家系的种子获得的，即受精后7周（90.9%）和受精后9周（91.2%）。忠南14、15和全北29家系在受精后10周没有反应（0%）。在萌发过程中，忠南11（44.0%）或忠南15（43.5%）获得上胚轴形成的最高频率，忠南11显示出胚根形成的最高速率（26.1%）。在上胚轴（43.5%）和胚根（26.1%）形成方面反应最灵敏的种子家系是忠南11。将20株幼苗移栽到珍珠岩：泥炭藓：蛭石（1:1:1）的土壤混合物中，8株植株在田间存活。
