Tian H Q, Russell S D
Department of Botany and Microbiology, University of Oklahoma, 73019, Norman, OK, USA.
Plant Cell Rep. 1997 Jun;16(9):657-661. doi: 10.1007/BF01275510.
This research is part of an attempt to establish an in vitro fertilization system in tobacco to aid in understanding mechanisms of fertilization. Fusions of isolated male and female gametes were induced in a polyethylene glycol solution. Fusion appears similar to that in maize. One nuclear division of both an unfertilized egg cell and a synergid was induced in KM8p medium with 1 mg/l 2,4-dichlorophenoxyacetic acid in a microchamber culture; one cellular division of the egg cell was also induced in the same medium in solid-drop culture. The osmolality of suspension culture feeder cells was critical for the development of these cells. These results indicate that in vitro fertilization is possible in tobacco, which would be the first such system in dicots.
本研究是建立烟草体外受精系统以辅助理解受精机制的尝试的一部分。在聚乙二醇溶液中诱导分离的雄配子和雌配子融合。融合过程看起来与玉米中的相似。在含有1毫克/升2,4-二氯苯氧乙酸的KM8p培养基中，于微室培养中诱导未受精卵细胞和助细胞进行一次核分裂；在固体滴培养的相同培养基中也诱导卵细胞进行一次细胞分裂。悬浮培养饲养细胞的渗透压对这些细胞的发育至关重要。这些结果表明烟草中体外受精是可能的，这将是双子叶植物中的首个此类系统。
