Maruyama E, Kinoshita I, Ishii K, Ohba K, Saito A
Institute of Agriculture and Forestry, University of Tsukuba, Tennodai 1-1-1, 305, Tsukuba, Ibaraki, Japan.
Bio-Resources Technology Division, Forestry and Forest Products Research Institute, Tuskuba Norinkenkyu Danchi-Nai, PO Box 16, 305, Ibaraki, Japan.
Plant Cell Rep. 1997 Mar;16(6):393-396. doi: 10.1007/BF01146780.
Germplasm conservation of the tropical forest trees,Cedrela odorata L.,Guazuma crinita Mart., andJacaranda mimosaefolia D. Don., at above-freezing temperatures following alginate-bead encapsulation was attempted. Shoot tips excised from in vitro plantlets were encapsulated in calcium-alginate beads and stored on different substrates at 12, 20, and 25 °C. Percent viability when encapsulated shoot tips were stored on substrate containing only water solidified with 1% (wt/vol) agar was 80% after 12 months at 12°C forC. odorata, 90% after 12 months at 25°C forG. crinita, and 70% after 6 months at 20°C forJ. mimosaefolia.
尝试在高于冰点的温度下，对热带森林树木洋椿（Cedrela odorata L.）、多毛瓜苏马（Guazuma crinita Mart.）和蓝花楹（Jacaranda mimosaefolia D. Don.）进行藻酸盐珠包封后的种质保存。从离体苗上切下的茎尖被包封在钙藻酸盐珠中，并在12、20和25℃下保存在不同的基质上。当包封的茎尖保存在仅含有用1%（重量/体积）琼脂固化的水的基质上时，洋椿在12℃下保存12个月后的存活率为80%，多毛瓜苏马在25℃下保存12个月后的存活率为90%，蓝花楹在20℃下保存6个月后的存活率为70%。
