通过固定在海藻酸钙凝胶珠中的平卧茄细胞提高东莨菪苷的产量。

Enhanced production of scopolin bySolanum aviculare cells immobilised within Ca-alginate gel beads.

作者信息

Roisin C, Gillet-Manceau F, Saucedo J E Nava, Fliniaux M, Jacquin-Dubreuil A, Barbotin J -N

机构信息

Laboratoire de Génie Cellulaire (EA 906), Université de Picardie Jules Verne, Faculté des Sciences, 33, rue St.-Leu, F-80039, Amiens Cedex, France.

Laboratoire de Pharmacognosie et Phytotechnologie (EA 908), Université de Picardie Jules Verne, Faculté de Pharmacie, 1, rue des Louvels, F-80037, Amiens Cedex 1, France.

出版信息

Plant Cell Rep. 1997 Feb;16(5):349-353. doi: 10.1007/BF01088295.

DOI:10.1007/BF01088295

PMID:30727677

Abstract

Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour ofS. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics ofSolanum aviculare cells immobilised within Ca-alginate beads were notably different from those of suspended cells. ImmobilisedS. aviculare have accumulated scopolin (up to 120 μg·g FWB) within beads and excreted it into the culture medium (up to 8 μg·g FWB). Contrary to suspended cells which have accumulated only traces of this metabolite within intracellular compartments (1 μg·g FWB), no scopolin has been found into the culture medium.

摘要

通过改变钙（0.1至1.5M）和海藻酸盐（1至1.5%）的浓度获得了不同的基质，用于研究固定化参数对鸟趾状石龙尾行为的影响。已观察到细胞生长、细胞释放以及东莨菪素产生和排泄的显著调节。固定在钙藻酸盐珠中的鸟趾状茄细胞的生理和形态特征与悬浮细胞明显不同。固定化的鸟趾状石龙尾在珠内积累了东莨菪素（高达120μg·g FWB）并将其排泄到培养基中（高达8μg·g FWB）。与仅在细胞内区室中积累痕量这种代谢物（1μg·g FWB）的悬浮细胞相反，在培养基中未发现东莨菪素。