一种为免疫组织学制备两栖动物胚胎切片的新方法。

A new method to prepare sections from amphibian embryos for immunohistology.

作者信息

Hausen P

机构信息

Max-Planck-Institut für Entwicklungsbiologie, Abteilung Zellbiologie, Tübingen, Bundesrepublik Deutschland.

出版信息

Z Naturforsch C J Biosci. 1988 Sep-Oct;43(9-10):765-8. doi: 10.1515/znc-1988-9-1021.

DOI:10.1515/znc-1988-9-1021

PMID:3072790

Abstract

The method involves impregnation of fixed embryos with a solution of plexiglass. The solvent is evaporated slowly. Blocks of plexiglass containing the specimen are thus formed. Sections (1-2 microns) are stretched and attached by an unconvential method. Immunohistological techniques allow antigen localization in a combination of high resolution, excellent quality of the sections and low background.

摘要

该方法包括用有机玻璃溶液浸渍固定好的胚胎。溶剂缓慢蒸发。由此形成含有标本的有机玻璃块。（1 - 2微米厚的）切片通过一种非常规方法进行拉伸和附着。免疫组织学技术能够在高分辨率、切片质量优良且背景低的条件下实现抗原定位。