非洲爪蟾凝集素定位于非洲爪蟾卵母细胞、卵和胚胎的多个部位。
Xenopus laevis lectin is localized at several sites in Xenopus oocytes, eggs, and embryos.
作者信息
Roberson M M, Barondes S H
出版信息
J Cell Biol. 1983 Dec;97(6):1875-81. doi: 10.1083/jcb.97.6.1875.
Abstract
The endogenous lectin of Xenopus laevis oocytes, unfertilized eggs, and blastula-stage embryos was immunohistochemically localized using a highly specific antiserum. Each tissue was examined with several techniques, including paraformaldehyde or glutaraldehyde fixation, frozen or plastic sections, and immunofluorescence or immunoperoxidase staining. In oocytes and unfertilized eggs, lectin was detected in association with yolk platelets, cortical granules, and the vitelline envelope. In embryos, cortical granules had disappeared and lectin was found in the cleavage furrows between the embryonic cells. The distribution of the lectin suggests that it plays more than one role in this developing system.
摘要
使用高度特异性抗血清对非洲爪蟾卵母细胞、未受精卵和囊胚期胚胎的内源性凝集素进行免疫组织化学定位。对每个组织采用了多种技术进行检查，包括多聚甲醛或戊二醛固定、冰冻或塑料切片以及免疫荧光或免疫过氧化物酶染色。在卵母细胞和未受精卵中，检测到凝集素与卵黄小板、皮质颗粒和卵黄膜相关。在胚胎中，皮质颗粒消失，在胚胎细胞之间的分裂沟中发现了凝集素。凝集素的分布表明它在这个发育系统中发挥不止一种作用。
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