Saravanan Chinnashanmugam, Kavitake Digambar, Kandasamy Sujatha, Devi Palanisamy Bruntha, Shetty Prathapkumar Halady
Department of Food Science and Technology, Pondicherry University, Pondicherry, 605014 India.
J Food Sci Technol. 2019 Jan;56(1):159-166. doi: 10.1007/s13197-018-3469-3. Epub 2018 Nov 8.
Exopolysaccharide (EPS) produced from KC117496 isolated from the naturally fermented batter has been reported earlier. Here, study aimed to optimize the carbon source to enhance the yield of EPS production, partial characterization and antioxidant activity of α-d-glucan EPS. Among different disaccharides (sucrose, maltose, lactose) and monosaccharides (glucose, galactose and fructose), combination of sucrose and glucose at 2% showed highest EPS production of 4.55 g/L in MRS medium. The molecular weight was identified as ~ 4.428 × 10 kDa with MALDI-TOF mass spectroscopy. 1D and 2D NMR results exhibited the presence of α-1-6 and α-1-3 linked glucose revealed EPS as a glucan. Antioxidant properties of glucan (500 µg/mL) revealed the significant oxidation alleviation potential such as DPPH (74%) and Hydroxyl radical activity (97.8%), whereas metal chelating activity (70%) was lower as compare to control standard. These characteristics of glucan EPS reveals its potential application in food and pharmaceutical industry.
先前已有报道从自然发酵面糊中分离出的 KC117496 产生的胞外多糖（EPS）。在此，研究旨在优化碳源以提高 α-d-葡聚糖 EPS 的产量、进行部分表征并测定其抗氧化活性。在不同的二糖（蔗糖、麦芽糖、乳糖）和单糖（葡萄糖、半乳糖和果糖）中，2% 的蔗糖和葡萄糖组合在 MRS 培养基中显示出最高的 EPS 产量，为 4.55 g/L。通过基质辅助激光解吸电离飞行时间质谱法确定分子量约为 4.428×10 kDa。一维和二维核磁共振结果显示存在 α-1-6 和 α-1-3 连接的葡萄糖，表明 EPS 是一种葡聚糖。葡聚糖（500 µg/mL）的抗氧化特性显示出显著的氧化缓解潜力，如 DPPH（74%）和羟基自由基活性（97.8%），而与对照标准相比，金属螯合活性（70%）较低。葡聚糖 EPS 的这些特性揭示了其在食品和制药行业的潜在应用。
