Kricka L J
Department of Pathology, Hospital of the University of Pennsylvania, Philadelphia 19104-4283.
Anal Biochem. 1988 Nov 15;175(1):14-21. doi: 10.1016/0003-2697(88)90354-5.
Recent advances in the analytical applications of bacterial and firefly luciferases and firefly luciferin are reviewed. Luciferases have been used in soluble and immobilized/co-immobilized forms in assays for a variety of enzymes, substrates, and cofactors. The firefly luciferase reaction forms the basis of rapid microbiological tests which have found application in susceptibility testing, detection of bacteriuria, activated sludge analysis, and food testing. Rapid microbiological assays are also possible using bacteriophages containing the lux genes from Virbrio harveyi. Both the firefly and the bacterial luciferase reaction have been applied in immunoassay and DNA probe assays and the firefly luciferin phosphate substrate for alkaline phosphatase labels has proven particularly successful.
本文综述了细菌荧光素酶、萤火虫荧光素酶及萤火虫荧光素在分析应用方面的最新进展。荧光素酶已以可溶形式以及固定化/共固定化形式用于多种酶、底物和辅因子的检测。萤火虫荧光素酶反应构成了快速微生物检测的基础，该检测已应用于药敏试验、菌尿检测、活性污泥分析及食品检测。使用含有哈维弧菌lux基因的噬菌体也可进行快速微生物检测。萤火虫荧光素酶反应和细菌荧光素酶反应均已应用于免疫分析和DNA探针分析，且已证明用于碱性磷酸酶标记的萤火虫荧光素磷酸底物特别成功。
