Jacouton Elsa, Torres Maravilla Edgar, Boucard Anne-Sophie, Pouderous Nicolas, Pessoa Vilela Ana Paula, Naas Isabelle, Chain Florian, Azevedo Vasco, Langella Philippe, Bermúdez-Humarán Luis G
Micalis Institute, AgroParisTech, INRA, Université Paris-Saclay, Jouy-en-Josas, France.
Instituto de Ciências Biológicas, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Front Microbiol. 2019 Jan 23;9:3355. doi: 10.3389/fmicb.2018.03355. eCollection 2018.
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine produced by T cells that participates and contributes in host defense and autoimmune disease. We have recently reported antitumor properties of the probiotic strain of BL23 in mice and T cells was shown to play an important role in this beneficial effect. In order to better understand the role of IL-17A in cancer, we constructed a recombinant strain of producing this cytokine and we determined its biological activity in: (i) a bioassay test for the induction of IL-6 production by murine fibroblasts 3T3 L1 cells line and (ii) in a mouse allograft model of human papilloma virus (HPV)-induced cancer. Our data show that recombinant produces and efficiently secretes biologically active IL-17A cytokine. Interestingly, ∼26% of mice intranasally treated with -IL-17A and challenged with TC-1 cells remained tumor free over the experiment, in contrast to control mice treated with the wild type strain of which developed 100% of aggressive tumors. In addition, the median size of the ∼74% tumor-bearing mice treated with recombinant -IL-17A, was significantly lower than mice treated with -wt. Altogether, our results demonstrate that intranasal administration with secreting IL-17A results in a partial protection against TC-1-induced tumors in mice, confirming antitumor effects of this cytokine in our cancer model.
白细胞介素-17A（IL-17A）是一种由T细胞产生的促炎细胞因子，参与宿主防御和自身免疫性疾病并发挥作用。我们最近报道了益生菌菌株BL23在小鼠体内的抗肿瘤特性，并且发现T细胞在这种有益作用中发挥重要作用。为了更好地理解IL-17A在癌症中的作用，我们构建了一种产生这种细胞因子的重组菌株，并在以下方面确定了其生物学活性：（i）通过小鼠成纤维细胞3T3 L1细胞系诱导IL-6产生的生物测定试验，以及（ii）在人乳头瘤病毒（HPV）诱导的癌症的小鼠同种异体移植模型中。我们的数据表明，重组菌株产生并有效分泌具有生物活性的IL-17A细胞因子。有趣的是，在实验过程中，约26%经鼻内给予-IL-17A并接种TC-1细胞的小鼠没有发生肿瘤，相比之下，用野生型菌株处理的对照小鼠100%发生侵袭性肿瘤。此外，用重组-IL-17A处理的约74%荷瘤小鼠的肿瘤中位大小明显低于用-wt处理的小鼠。总之，我们的结果表明，经鼻内给予分泌IL-17A的菌株可使小鼠对TC-1诱导的肿瘤产生部分保护作用，证实了这种细胞因子在我们的癌症模型中的抗肿瘤作用。
