Gould S J, Subramani S
Department of Biology, University of California, San Diego, La Jolla 92093.
Anal Biochem. 1988 Nov 15;175(1):5-13. doi: 10.1016/0003-2697(88)90353-3.
The unique properties of firefly luciferase and the cloning of the gene for this enzyme have spawned a number of novel applications of this protein. We summarize a few of these applications including its use as a reporter gene, as a model for the study of protein import into peroxisomes, and as a component of a heterologous gene expression system.
萤火虫荧光素酶的独特性质以及该酶基因的克隆催生了这种蛋白质的许多新应用。我们总结其中一些应用,包括其作为报告基因的用途、作为研究蛋白质导入过氧化物酶体的模型以及作为异源基因表达系统的一个组成部分。