Jiao Keyuan, Li Xin, Su Shihao, Guo Wuxiu, Guo Yafang, Guan Yining, Hu Zhubing, Shen Zhenguo, Luo Da
1Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, China.
2College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Hortic Res. 2019 Feb 1;6:23. doi: 10.1038/s41438-018-0088-0. eCollection 2019.
Many studies suggest that there are distinct regulatory processes controlling compound leaf development in different clades of legumes. Loss of function of the () orthologs results in a reduction of leaf complexity to different degrees in inverted repeat-lacking clade (IRLC) and non-IRLC species. To further understand the role of orthologs and the molecular mechanism in compound leaf development in non-IRLC plants, we studied leaf development in () mutant, a classical mutant of mungbean ( L.), which showed a complete conversion of compound leaves into simple leaves. Our analysis revealed that encoded the mungbean LFY ortholog (VrLFY) and played a significant role in leaf development. In situ RNA hybridization results showed that -like genes were expressed in compound leaf primordia in mungbean. Furthermore, increased leaflet number in () mutants was demonstrated to depend on the function of and genes in mungbean. Our results suggested that is a key factor coordinating distinct processes in the control of compound leaf development in mungbean and its related non-IRLC legumes.
许多研究表明，在豆科植物的不同分支中存在控制复叶发育的不同调控过程。（）直系同源基因功能的丧失导致在缺乏反向重复序列的分支（IRLC）和非IRLC物种中叶片复杂性不同程度降低。为了进一步了解直系同源基因在非IRLC植物复叶发育中的作用及其分子机制，我们研究了绿豆（Vigna radiata L.）的经典突变体（）突变体中的叶片发育，该突变体表现出复叶完全转变为单叶。我们的分析表明，（）编码绿豆LFY直系同源基因（VrLFY）并在叶片发育中起重要作用。原位RNA杂交结果表明，类似（）的基因在绿豆的复叶原基中表达。此外，已证明（）突变体中叶数增加依赖于绿豆中（）和（）基因的功能。我们的结果表明，（）是协调绿豆及其相关非IRLC豆科植物复叶发育控制中不同过程的关键因素。
