Li Zhongfeng, Jiang Lingxue, Ma Yansong, Wei Zhongyan, Hong Huilong, Liu Zhangxiong, Lei Jinhui, Liu Ying, Guan Rongxia, Guo Yong, Jin Longguo, Zhang Lijuan, Li Yinghui, Ren Yulong, He Wei, Liu Ming, Htwe Nang Myint Phyu Sin, Liu Lin, Guo Bingfu, Song Jian, Tan Bing, Liu Guifeng, Li Maiquan, Zhang Xianli, Liu Bo, Shi Xuehui, Han Sining, Hua Sunan, Zhou Fulai, Yu Lili, Li Yanfei, Wang Shuang, Wang Jun, Chang Ruzhen, Qiu Lijuan
National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
J Integr Plant Biol. 2017 Jan;59(1):60-74. doi: 10.1111/jipb.12505.
Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)-induced soybean (Glycine max) population, consisting of 21,600 independent M lines, was developed. Over 1,000 M families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to MinnGold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene (ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.
诱变群体为引入植物变异和鉴定基因功能提供了重要材料。本研究构建了一个由21,600个独立M系组成的甲基磺酸乙酯（EMS）诱变大豆（Glycine max）群体。鉴定出1000多个M家系，其种子成分、种子形状、植株形态和成熟度等多种异常表型在不同环境和世代中稳定表达。对该群体的表型分析鉴定出一个黄色素突变体gyl，其叶绿素（Chl）含量显著降低，叶绿体发育异常。序列分析表明，gyl与MinnGold等位，Mg螯合酶亚基基因（ChlI1a）中不同的单核苷酸多态性变异导致叶片呈金黄色。开发了一种酶切扩增多态性序列标记，可应用于大豆育种中金黄色表型的标记辅助选择。我们表明，新构建的大豆EMS突变体群体在大豆功能基因组学研究和遗传改良方面具有潜力。
