Kravchenko V I
Ukr Biokhim Zh (1978). 1978 Mar-Apr;50(2):180-3.
The corticosteroids biosynthesis in adrenals with administration of o,p'-DDD in doses of 40, 100, 200 mg/kg was studied for two weeks in experiments on chickens. The corticosteroids biosynthesis was judged by formation of corticosterone, hydrocortisone and desoxycorticosterone by 2% adrenal homogenates from the precursors (pregnelonone and 11-hydroxyprogesterone) introduced into the incubation medium. The formed corticosteriods were isolated chromatographically, then they were identified and quantitatively determined by spectrophotometry following the reaction with tetrazolium blue. The data of the studies performed by thin-layer chromatography show the inhibition of the 11-beta-hydroxylizing system with administration of o,p'-DDD in a dose of 50 mg/kg and stimulation of 21-hydroxylation of steroids. When introducing the inhibitor in a larger amount, most enzymatic systems participating in the process of the corticosteroids biosynthesis are inhibited.
在鸡的实验中，研究了连续两周给予剂量为40、100、200mg/kg的邻对滴滴滴（o,p'-DDD）时肾上腺中皮质类固醇的生物合成。通过将前体物质（孕烯醇酮和11-羟孕酮）引入孵育培养基中，由2%的肾上腺匀浆生成皮质酮、氢化可的松和脱氧皮质酮来判断皮质类固醇的生物合成。生成的皮质类固醇通过色谱法分离，然后在与四氮唑蓝反应后，通过分光光度法进行鉴定和定量测定。薄层色谱法的研究数据表明，给予50mg/kg剂量的邻对滴滴滴会抑制11-β-羟化系统，并刺激类固醇的21-羟化。当引入更大剂量的抑制剂时，参与皮质类固醇生物合成过程的大多数酶系统都会受到抑制。
