Department of Chemical Engineering, Federal University of Santa Maria, 1000, Roraima Avenue, Santa Maria, 97105-900, Brazil.
Department of Crop Protection, Federal University of Santa Maria, 1000, Roraima Avenue, Santa Maria, 97105-900, Brazil.
Appl Biochem Biotechnol. 2019 Aug;188(4):914-926. doi: 10.1007/s12010-019-02966-7. Epub 2019 Feb 7.
The performance of lipase NS 40116, a novel and promising soluble enzyme obtained from modified Thermomyces lanuginosus microorganism, was investigated in the production of biodiesel (fatty acid methyl esters-FAME) by hydroesterification. In order to investigate the potential of the biocatalyst in its soluble form, this work reports the effect of water content and enzyme load, as well as the recovery and reuse of the biocatalyst. A FAME yield of 94.30% after 12 h was achieved at 35 °C by combining 0.50 wt% of lipase, 15 wt% of water, and a methanol:oil molar ratio of 4.5:1. The analysis of the time course reaction suggests that the triacylglycerides (TAGs) are hydrolyzed by the enzyme in a first step, generating free fatty acids (FFAs), followed by the esterification of these FFAs into FAME. In relation to the reusability assays, the lipase kept approximately 90% of its catalytic activity after five cycles of reuse. In this context, the findings of this study demonstrate that lipase NS 40116 can efficiently catalyze hydroesterification reactions under mild conditions, arising as a competitive alternative for biodiesel synthesis.
新型可溶脂肪酶 NS 40116 的酶学性质及其在生物柴油合成中的应用
从经过改良的嗜热真菌Thermomyces lanuginosus 微生物中获得的新型可溶脂肪酶 NS 40116 的性能，通过水相酯化法生产生物柴油（脂肪酸甲酯-FAME）的过程中进行了研究。为了研究该生物催化剂在可溶形式下的潜力，本工作报告了含水量和酶负载量以及生物催化剂的回收和再利用的影响。在 35°C 下，当脂肪酶用量为 0.50wt%、水用量为 15wt%、甲醇:油摩尔比为 4.5:1 时，经过 12 h 的反应，可获得 94.30%的 FAME 产率。反应时间进程的分析表明，三酰基甘油(TAGs)首先被酶水解，生成游离脂肪酸(FFAs)，然后这些 FFAs 酯化生成 FAME。就可重复使用性试验而言，脂肪酶在重复使用五轮后保持了约 90%的催化活性。在这种情况下，本研究的结果表明，脂肪酶 NS 40116 可以在温和条件下有效地催化水相酯化反应，是生物柴油合成的一种有竞争力的替代方法。
