通过蛋白质免疫印迹法和免疫沉淀法分析信号通路

Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation.

作者信息

Marino Silvia, Idris Aymen I

机构信息

Division Hematology Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.

Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK.

出版信息

Methods Mol Biol. 2019;1914:131-143. doi: 10.1007/978-1-4939-8997-3_7.

DOI:10.1007/978-1-4939-8997-3_7

PMID:30729463

Abstract

This chapter describes the analysis of signaling pathways in bone cells by the use of western blotting and immunoprecipitation, including a step-by-step guide to cell culture techniques, cellular and subcellular fractionation, protein isolation, purification, measurement, electrophoretic transfer, and detection.

摘要

本章介绍了利用蛋白质免疫印迹法和免疫沉淀法分析骨细胞中的信号通路，包括细胞培养技术、细胞及亚细胞分级分离、蛋白质分离、纯化、测量、电泳转移和检测的分步指南。