骨细胞中转录调控的分析

Analysis of Transcriptional Regulation in Bone Cells.

作者信息

Jin Huilin, Sophocleous Antonia, Azfer Asim, Ralston Stuart H

机构信息

South West Thames Regional Genetics Laboratory, St George's Hospital, London, UK.

Kennedy Institute of Rheumatology, London, UK.

出版信息

Methods Mol Biol. 2019;1914:145-167. doi: 10.1007/978-1-4939-8997-3_8.

DOI:10.1007/978-1-4939-8997-3_8

PMID:30729464

Abstract

Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay, five commonly used methods for studying and altering gene transcription.

摘要

转录是一个调节RNA合成速率的过程。在此，我们描述了进行启动子-报告基因检测、电泳迁移率变动分析、染色体构象捕获（3C）分析、染色质免疫沉淀分析和CRISPR-Cas9分析的技术，这是研究和改变基因转录的五种常用方法。