Moukengue Brice, Amiaud Jérôme, Jacques Camille, Charrier Céline, Ory Benjamin, Lamoureux Francois
INSERM, UMR1238, Bone Sarcoma and Remodeling of Calcified Tissues, Université de Nantes, Nantes Atlantique Universités, Nantes, France.
Methods Mol Biol. 2019;1914:169-196. doi: 10.1007/978-1-4939-8997-3_9.
The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes .
本章的目的是描述一种使用逆转录和定量聚合酶链反应（RT-qPCR）来评估骨细胞或组织中基因表达和微小RNA（miRNA）的方法，以及一种在福尔马林固定石蜡包埋（FFPE）小鼠骨组织上评估显色原位杂交（CISH）的方法，该方法使用双地高辛（DIG）锁核酸（LNA™）探针来检测DNA和mRNA转录本。
