Javaheri Behzad, Bravenboer Nathalie, Bakker Astrid D, van der Veen Albert, de Souza Roberto Lopes, Saxon Leanne, Pitsillides Andrew A
Skeletal Biology Group, Comparative Biomedical Sciences, The Royal Veterinary College, London, UK.
Department Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands.
Methods Mol Biol. 2019;1914:369-390. doi: 10.1007/978-1-4939-8997-3_22.
The skeleton fulfils its mechanical functions through structural organization and material properties of individual bones. It is stated that both cortical and trabecular morphology and mass can be (re)modelled in response to changes in mechanical strains engendered by load-bearing. To address this, animal models that enable the application of specific loads to individual bones have been developed. These are useful in defining how loading modulates (re)modeling and allow examination of the mechanisms that coordinate these events. This chapter describes how to apply mechanical loading to murine bones through points of articulation, which allows changes in endosteal, periosteal as well as trabecular bone to be revealed at multiple hierarchies, by a host of methodologies, including double fluorochrome labeling and computed tomography.
骨骼通过单个骨骼的结构组织和材料特性来实现其机械功能。有研究表明，皮质骨和小梁骨的形态及质量都可以根据承重产生的机械应变变化进行（重新）塑造。为了研究这一点，已经开发出了能够对单个骨骼施加特定负荷的动物模型。这些模型有助于确定负荷如何调节（重新）塑造过程，并能对协调这些过程的机制进行研究。本章描述了如何通过关节点对小鼠骨骼施加机械负荷，这可以通过包括双荧光染料标记和计算机断层扫描在内的一系列方法，在多个层次上揭示骨内膜、骨膜以及小梁骨的变化。
