骨细胞凋亡的研究技术

Techniques for the Study of Apoptosis in Bone.

作者信息

Mann Val, Noble Brendon

机构信息

Faculty of Medicine and Dentistry, University of Plymouth, Plymouth, UK.

School of Life Sciences, University of Westminster, London, UK.

出版信息

Methods Mol Biol. 2019;1914:451-465. doi: 10.1007/978-1-4939-8997-3_26.

DOI:10.1007/978-1-4939-8997-3_26

PMID:30729482

Abstract

Osteocyte apoptosis has been associated with a number of clinical conditions in bone and with the targeted turnover of specific skeletal areas. There has been great interest in the identification of the mechanisms by which apoptosis is regulated in bone and in the biological role that this process plays in bone metabolism and associated bone disease or loss of structural integrity. Here we describe several methods for the detection of apoptosis in bone sections and in bone cell cultures.

摘要

骨细胞凋亡与多种骨骼临床病症以及特定骨骼区域的靶向更新有关。人们对确定骨骼中凋亡调节机制以及该过程在骨代谢、相关骨病或结构完整性丧失中所起的生物学作用非常感兴趣。在此，我们描述了几种检测骨切片和骨细胞培养物中凋亡的方法。