剪接体中的 RNA：冷冻电镜结构的启示。
RNAs in the spliceosome: Insight from cryoEM structures.
机构信息
Department of Biochemistry and Molecular Genetics, Anschutz Medical Campus, University of Colorado Denver, Aurora, Colorado.
出版信息
Wiley Interdiscip Rev RNA. 2019 May;10(3):e1523. doi: 10.1002/wrna.1523. Epub 2019 Feb 6.
Abstract
Pre-mRNA splicing is catalyzed by the spliceosome, a multimegadalton RNA-protein complex. The spliceosome undergoes dramatic compositional and conformational changes through the splicing cycle, forming at least 10 distinct complexes. Recent high-resolution cryoEM structures of various spliceosomal complexes revealed unprecedented details of this large molecular machine. This review highlights insight into the structure and function of the spliceosomal RNA components obtained from these new structures, with a focus on the yeast spliceosome. This article is categorized under: RNA Processing > Splicing Mechanisms RNA Structure and Dynamics > RNA Structure, Dynamics, and Chemistry RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes.
摘要
前体 mRNA 剪接由剪接体催化，这是一个包含多个大亚基的 RNA-蛋白复合物。剪接体通过剪接循环经历剧烈的组成和构象变化，形成至少 10 种不同的复合物。最近对各种剪接体复合物的高分辨率冷冻电镜结构揭示了这个大型分子机器的前所未有的细节。这篇综述重点介绍了从这些新结构中获得的剪接体 RNA 成分的结构和功能的见解，特别关注酵母剪接体。本文属于以下分类：RNA 加工 > 剪接机制 RNA 结构和动态学 > RNA 结构、动态和化学 RNA 与蛋白质和其他分子的相互作用 > RNA-蛋白复合物。
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