Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.
Bauman Moscow State Technical University, Moscow, Russia.
J Biomed Opt. 2019 Feb;24(2):1-5. doi: 10.1117/1.JBO.24.2.027001.
We applied terahertz (THz)-pulsed spectroscopy to study ex vivo the refractive index and absorption coefficient of human brain gliomas featuring different grades, as well as perifocal regions containing both intact and edematous tissues. Glioma samples from 26 patients were considered and analyzed according to further histological examination. In order to fix tissues for the THz measurements, we applied gelatin embedding, which allows for sustaining their THz response unaltered, as compared to that of the freshly excised tissues. We observed a statistical difference between the THz optical constants of intact tissues and gliomas of grades I to IV, while the response of edema was similar to that of tumor. The results of this paper justify a potential of THz technology in the intraoperative label-free diagnosis of human brain gliomas for ensuring the gross-total resection.
我们应用太赫兹（THz）脉冲光谱技术，对不同分级的人脑胶质瘤以及包含正常和水肿组织的瘤周区域的折射率和吸收系数进行了离体研究。根据进一步的组织学检查，对 26 名患者的胶质瘤样本进行了分析。为了固定用于太赫兹测量的组织，我们应用了明胶包埋，这使得它们的太赫兹响应与刚切除的组织相比保持不变。我们观察到正常组织与 I 至 IV 级胶质瘤的太赫兹光学常数之间存在统计学差异，而水肿的响应与肿瘤相似。本文的结果证明了太赫兹技术在人脑中进行胶质瘤的术中无标记诊断方面具有潜力，以确保实现大体全切除。
