On Slw, Brett B, Horan S, Erskine H, Lin S, Cornelius A J
a Department of Wine, Food and Molecular Biosciences , Lincoln University , Lincoln , New Zealand.
b Institute of Environmental Science and Research (ESR) , Christchurch , New Zealand.
N Z Vet J. 2019 May;67(3):134-137. doi: 10.1080/00480169.2019.1580167. Epub 2019 Mar 11.
To investigate the presence of Campylobacter spp. in captive kiwi (Apteryx spp.) and compare their genotypic profiles with those of human and animal origin, in order to assess their potential for zoonotic or zooanthroponotic transmission.
Conventional selective enrichment and filter-based isolation methods were applied to isolate Campylobacter spp. from fresh faecal samples from 12 North Island brown kiwi (Apteryx mantelli) and one great spotted kiwi (A. haastii), housed in one of five different areas in a kiwi sanctuary in Christchurch, New Zealand. Isolates were identified using multiplex PCR and 16S rRNA gene sequencing. High-resolution rapid genotyping using multiplex ligation-dependant probe amplification-based binary typing (MBiT) was applied and profiles compared with similar results from 2,165 Campylobacter spp. isolates contained in a database derived from human clinical, veterinary and environmental samples.
One isolate of C. jejuni, and one belonging to the C. lari phylogenetic group were recovered from faeces from two kiwi. High-resolution rapid genotyping by MBiT demonstrated these to be indistinguishable from isolates obtained previously from human cases of diarrhoea, and others from chicken, cattle, sheep and water.
These data provide evidence for potential zoonotic or zooanthroponotic transmission of Campylobacter spp. in kiwi with implications for management of birds kept in captivity. We believe this is the first formal report of C. jejuni and a C. lari-like organism in kiwi.
MBiT: Multiplex ligation-dependant probe amplification-based binary typing.
调查圈养几维鸟（几维属）中弯曲杆菌属的存在情况，并将其基因型谱与人类和动物来源的进行比较，以评估其人畜共患病或动物传人病传播的可能性。
采用传统的选择性富集和基于过滤的分离方法，从新西兰克赖斯特彻奇一个几维鸟保护区五个不同区域之一饲养的12只北岛褐几维鸟（褐几维）和1只大斑几维鸟（哈斯特几维）的新鲜粪便样本中分离弯曲杆菌属。使用多重聚合酶链反应（PCR）和16S核糖体RNA（rRNA）基因测序对分离株进行鉴定。应用基于多重连接依赖探针扩增的二元分型（MBiT）进行高分辨率快速基因分型，并将图谱与来自人类临床、兽医和环境样本的数据库中包含的2165株弯曲杆菌属分离株的类似结果进行比较。
从两只几维鸟的粪便中分别分离出一株空肠弯曲菌和一株属于海鸥弯曲菌系统发育组的菌株。通过MBiT进行的高分辨率快速基因分型表明，这些菌株与先前从人类腹泻病例以及从鸡、牛和羊及水中分离得到的菌株无法区分。
这些数据为弯曲杆菌属在几维鸟中潜在的人畜共患病或动物传人病传播提供了证据，对圈养鸟类的管理具有重要意义。我们认为这是关于几维鸟中空肠弯曲菌和一种类似海鸥弯曲菌的生物体的首次正式报告。
MBiT：基于多重连接依赖探针扩增的二元分型
