Yang Wen, Li Zhen, Lu Zhengyi, Yu Jing, Huo Yanyan, Man Baoyuan, Pan Jie, Si Haipeng, Jiang Shouzhen, Zhang Chao
Opt Express. 2019 Feb 4;27(3):3000-3013. doi: 10.1364/OE.27.003000.
In recent years, biomaterials have increasingly attracted attention on surface-enhanced Raman spectroscopy (SERS) due to their well Raman performance while metal particles are combined with biological substrates. Therefore, we propose an environmentally friendly substrate based on silver-plated cicada wings with seamless graphene layer (Gr-AgNPs-C.w.), which can be prepared with a simple and inexpensive method. Compared with AgNPs-C.w., Gr-AgNPs-C.w. hybrids show better SERS performance with high sensitivity, good uniformity and good stability with R6G detection. The minimum detected concentration can reach 10 M, and the value of R can reach 0.996, respectively. Theoretical simulation demonstrates the situation of electromagnetic field through COMSOL software. In addition, due to the affinity of graphene for biomolecules, we can successfully detect the DNA biomolecules through a simple process. Therefore, this cheap and efficient natural SERS substrate has great potential for a considerable number of biochemical SERS applications and can broaden the way in which multiple SERS platforms derived from other natural materials are prepared.
近年来，生物材料在与生物底物结合金属颗粒时因其良好的拉曼性能而越来越受到表面增强拉曼光谱（SERS）的关注。因此，我们提出了一种基于镀银蝉翼并带有无缝石墨烯层（Gr-AgNPs-C.w.）的环保基底，它可以通过简单且廉价的方法制备。与AgNPs-C.w.相比，Gr-AgNPs-C.w.杂化物在检测R6G时表现出更好的SERS性能，具有高灵敏度、良好的均匀性和良好的稳定性。最低检测浓度分别可达到10 M，R值可达到0.996。理论模拟通过COMSOL软件展示了电磁场的情况。此外，由于石墨烯对生物分子的亲和力，我们可以通过一个简单的过程成功检测DNA生物分子。因此，这种廉价且高效的天然SERS基底在大量生化SERS应用中具有巨大潜力，并且可以拓宽源自其他天然材料的多种SERS平台的制备方式。
