在连续流动培养系统中，由海带（海带目，褐藻纲）原生质体进行植株再生

Plant regeneration from protoplasts of Laminaria japonica Areschoug (Laminariales, Phaeophyceae) in a continuous-flow culture system.

作者信息

Sawabe T, Ezura Y, Yamamoto H

机构信息

Laboratory of Microbiology, Department of Food Science and Technology, Faculty of Fisheries, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041, Japan Fax no.: +81-138-40-5569 E-mail:

出版信息

Plant Cell Rep. 1997 Dec;17(2):109-112. doi: 10.1007/s002990050361.

DOI:10.1007/s002990050361

PMID:30732397

Abstract

A continuous-flow culture system was developed for culturing Laminaria japonica protoplasts. Protoplasts were settled on 5-µm pore size nylon mesh fixed inside a 50-ml plastic syringe, and cultured in Provasoli's enriched seawater with iodine medium with a gentle upward flow generated by a peristaltic pump. In the culture system, 50% of the protoplasts regenerated their cell wall within 24 hours and almost all protoplasts regenerated a cell wall after 3 days culture. After cell wall regeneration, a number of cells divided and regenerated into sheet-shaped thalli. The thalli transferred to a tissue culture flask developed into sporophyte-like plantlets within 1 month. Plantlets then differentiated into blade, stipe, and holdfast, with a proper mucilage canal.

摘要

开发了一种用于培养海带原生质体的连续流动培养系统。原生质体沉降在固定于50毫升塑料注射器内的孔径为5微米的尼龙网上，并在添加碘的普罗瓦索利富海水培养基中培养，通过蠕动泵产生缓慢向上的水流。在该培养系统中，50%的原生质体在24小时内再生细胞壁，培养3天后几乎所有原生质体都再生了细胞壁。细胞壁再生后，许多细胞分裂并再生为片状叶状体。转移到组织培养瓶中的叶状体在1个月内发育成类孢子体幼苗。幼苗随后分化出叶片、柄和固着器，并具有合适的黏液管。