葡萄（Vitis vinifera L.）卷须中体细胞胚胎发生及小植株的诱导

Induction of somatic embryogenesis and plantlets in tendrils of Vitis vinifera L.

作者信息

Salunkhe C K, Rao P S, Mhatre Minal

机构信息

Biotechnology Division and L & CM Section, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India Fax no.: +091-22-556-0750 E-mail:

出版信息

Plant Cell Rep. 1997 Nov;17(1):65-67. doi: 10.1007/s002990050353.

DOI:10.1007/s002990050353

PMID:30732422

Abstract

Somatic embryogenesis was observed in callus initiated from tendril explants of Vitis vinifera L. cvs. Thompson, Sonaka and Tas-e-Ganesh on Emershad and Ramming medium supplemented with 1 µM 6-benzylaminopurine. Low-frequency conversion to shoots was obtained in the third and fourth subculture on the same medium. Emerging shoots subsequently formed complete plantlets on liquid rooting medium containing 1 µM indole-3-acetic acid. The possible use of tendrils as a novel explant for somatic embryogenesis in grape is discussed.

摘要

在添加了1μM 6-苄基腺嘌呤的埃默沙德和拉明培养基上，从葡萄品种汤普森、索纳卡和塔斯-伊-加内什的卷须外植体诱导出的愈伤组织中观察到了体细胞胚胎发生。在同一培养基上进行第三次和第四次继代培养时，获得了低频率的芽转化。随后，长出的芽在含有1μM吲哚-3-乙酸的液体生根培养基上形成了完整的植株。本文讨论了卷须作为葡萄体细胞胚胎发生新外植体的可能性。