无籽葡萄（Vitis vinifera L.）未成熟胚胎的体细胞胚胎发生和植株发育。

Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.).

机构信息

USDA, ARS, HCRL, 2021 South Peach Avenue, 93727, Fresno, CA, USA.

出版信息

Plant Cell Rep. 1994 Nov;14(1):6-12. doi: 10.1007/BF00233289.

PMID:24194218

Abstract

Somatic embryo formation occurred from immature zygotic embryos within ovules of stenospermocarpic seedless grapes (Vitis vinifera L.), when cultured for two months on liquid Emershad/Ramming medium. Somatic embryos continued to proliferate after excision and transfer to Emershad/Ramming medium supplemented with 1 μM benzylaminopurine and 0.65% TC agar. Plant development from somatic embryos was influenced by genotype, medium, phase (liquid, agar), stage (torpedo, mature) and their interactions. Optimal plant development occurred on Woody Plant Medium supplemented with 1.5% sucrose + 1 μM benzylaminopurine + 0.3% activated charcoal and 0.65% TC agar.

摘要

无融合生殖胚状体的形成发生在珠心内未成熟的合子胚中，无核葡萄（Vitis vinifera L.）的珠心内未成熟的合子胚在液体 Emershad/Ramming 培养基中培养两个月后形成。体细胞胚在切除并转移到添加 1 μM 苄基氨基嘌呤和 0.65% TC 琼脂的 Emershad/Ramming 培养基中后继续增殖。体细胞胚的植物发育受基因型、培养基、阶段（液体、琼脂）、阶段（鱼雷形、成熟）及其相互作用的影响。最佳的植物发育发生在 Woody Plant 培养基中，该培养基补充了 1.5%蔗糖+1 μM 苄基氨基嘌呤+0.3%活性炭和 0.65% TC 琼脂。