Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan, PR China; Hubei Key Laboratory of Cardiology, Wuhan, PR China.
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan, PR China; Hubei Key Laboratory of Cardiology, Wuhan, PR China.
Biochem Biophys Res Commun. 2019 Mar 12;510(3):456-461. doi: 10.1016/j.bbrc.2019.01.129. Epub 2019 Feb 4.
Platelet, apart from its classic role of homeostasis, serves also as a crucial immune cell component that contributes to the aggravation of atherosclerosis. It has been reported that myocardial infarction (MI) triggers leukocytosis in the bone marrow and spleen, which accelerates post-MI atherosclerosis. However, it remains unclear whether thrombopoiesis is also enhanced after MI. Here, using flow cytometry and bone marrow whole-mount immunofluorescence staining combined with three-dimensional (3D) reconstruction, we for the first time demonstrated an enhanced thrombopoiesis and megakaryopoiesis in a mouse model of coronary artery ligation as a mimic of MI. We showed that MI leads to increasing number of peripheral platelets, as well as elevating number and larger size of bone marrow MKs. We also observed more proplatelets and fragmented MKs, and a closer spatial distribution of MK populations to the bone marrow vascular niche after MI. This study provides direct evidence that MI induces bone marrow megakaryocyte proliferation, maturation and platelet production. It opens a new scope that targeting platelet production might become a novel therapeutic approach for attenuating post-MI atherosclerosis.
血小板除了具有经典的稳态作用外，还作为一种关键的免疫细胞成分，有助于动脉粥样硬化的加重。据报道，心肌梗死（MI）会在骨髓和脾脏中引发白细胞增多，从而加速 MI 后的动脉粥样硬化。然而，目前尚不清楚 MI 后是否也会增强血小板生成。在这里，我们首次使用流式细胞术和骨髓全骨髓免疫荧光染色结合三维（3D）重建，在冠状动脉结扎的小鼠模型中证实了这种模拟 MI 的情况下血小板生成和巨核细胞生成的增强。我们发现 MI 导致外周血小板数量增加，骨髓 MK 的数量和大小增加。我们还观察到更多的前血小板和碎片化的 MK，以及 MI 后 MK 群体与骨髓血管巢之间的空间分布更加接近。这项研究提供了直接证据，证明 MI 诱导骨髓巨核细胞增殖、成熟和血小板生成。它开辟了一个新的领域，即靶向血小板生成可能成为减轻 MI 后动脉粥样硬化的一种新的治疗方法。
