Department of Bioscience, Shri Ram Group of College (SRGC), Muzaffarnagar 251001, India; Nanomedicine & Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
King Abdullah Institute for Nanotechnology, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.
J Trace Elem Med Biol. 2019 Mar;52:12-17. doi: 10.1016/j.jtemb.2018.11.003. Epub 2018 Nov 10.
Despite the extensive implication of nickel oxide nanoparticles (NiO-NPs) in different fields such as biomedical science and industrial manufacturing, their effect on human cancer cells has not been elucidated. In this study, we report a simple process for the preparation of NiO-NPs. X-ray diffraction and transmission electron microscopy were used to characterize the surface architecture and dimension of the synthesized NiO-NPs. The average diameter of the NiO-NPs was approximately 20-25 nm. We used two human colon cancer cell lines, HT-29 and SW620, to assess the nanoparticles' cytotoxicity. The MTT assay showed that the NiO-NPs reduced cell viability of HT-29 and SW620 cell lines. The results of inverted microscopy showed the highest cytotoxic activity with 600 μg/ml concentration of NiO-NPs on HT-29 cells. Western blot assay showed the downregulation of anti-apoptotic Bcl2 and Bcl-xL proteins in HT-29 cells treated with NiO-NPs. Moreover the results demonstrated the induction of PARP (Cleaved) in NiO-NPs treated HT-29 cells which are considered the marker of apoptosis. The NiO-NPs were not demonstrated bactericidal effect on six different bacterial strains tested, implying that the NiO-NPs may not perturb the human normal gut microbiome. The results have showed the promising application of the NiO-NPs in management of cancer in near future.
尽管氧化镍纳米粒子（NiO-NPs）在生物医学科学和工业制造等不同领域有广泛的应用，但它们对人类癌细胞的影响尚未阐明。在本研究中，我们报告了一种制备 NiO-NPs 的简单方法。X 射线衍射和透射电子显微镜用于表征合成的 NiO-NPs 的表面结构和尺寸。NiO-NPs 的平均直径约为 20-25nm。我们使用两种人结肠癌细胞系 HT-29 和 SW620 来评估纳米颗粒的细胞毒性。MTT 测定表明，NiO-NPs 降低了 HT-29 和 SW620 细胞系的细胞活力。倒置显微镜的结果显示，HT-29 细胞用 600μg/ml 浓度的 NiO-NPs 表现出最高的细胞毒性活性。Western blot 分析表明，NiO-NPs 处理的 HT-29 细胞中抗凋亡 Bcl2 和 Bcl-xL 蛋白下调。此外，结果表明 NiO-NPs 诱导了 PARP（切割）在 NiO-NPs 处理的 HT-29 细胞中，PARP（切割）被认为是细胞凋亡的标志物。NiO-NPs 对测试的六种不同细菌菌株没有表现出杀菌作用，这意味着 NiO-NPs 可能不会干扰人类正常的肠道微生物组。结果表明，NiO-NPs 在癌症管理方面具有广阔的应用前景。
