Department of Pesticide Sciences, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Jiangsu, China.
Pest Manag Sci. 2019 Oct;75(10):2663-2671. doi: 10.1002/ps.5371. Epub 2019 Mar 21.
Octopamine, the invertebrate counterpart of adrenaline and noradrenaline, regulates and modulates many physiological and behavioral processes in insects. It mediates its effects by binding to specific octopamine receptors, which belong to the superfamily of G-protein coupled receptors (GPCRs). The expression profiles of octopamine receptor genes have been well documented in different developmental stages and multiple tissue types in several different insect orders. However, little work has addressed this issue in Hemiptera.
In this study, we cloned four octopamine receptor genes from brown planthopper. The deduced amino acid sequences share high identity with other insect homologues and have the characteristic GPCRs domain architecture: seven transmembrane domains. These genes were expressed in all developmental stages and examined tissues. The expression of NlOA2B3 and NlOA3 was relatively higher in egg and first instar nymph stage than in other stages and other receptor genes. All of these receptor genes were more highly expressed in brain than other tissues.
The identification of octopamine receptor genes in this study will provide a foundation for investigating the diverse roles played by NlOARs and for exploring specific target sites for chemicals that control agricultural pests. © 2019 Society of Chemical Industry.
章鱼胺是昆虫体内肾上腺素和去甲肾上腺素的无脊椎动物对应物，它通过与特定的章鱼胺受体结合来调节和调制许多生理和行为过程，这些受体属于 G 蛋白偶联受体（GPCRs）超家族。在几个不同的昆虫目，章鱼胺受体基因的表达谱在不同的发育阶段和多种组织类型中都有很好的记录。然而，在半翅目昆虫中，很少有工作涉及这个问题。
在这项研究中，我们从褐飞虱中克隆了四个章鱼胺受体基因。推导的氨基酸序列与其他昆虫同源物具有高度的同一性，并具有特征性的 GPCRs 结构域架构：七个跨膜结构域。这些基因在所有发育阶段和检测组织中都有表达。NlOA2B3 和 NlOA3 的表达在卵和第一龄若虫期相对高于其他阶段和其他受体基因。所有这些受体基因在脑中的表达都高于其他组织。
本研究中章鱼胺受体基因的鉴定将为研究 NlOARs 所发挥的多种作用以及探索控制农业害虫的化学物质的特定靶标提供基础。 © 2019 化学工业协会。
