通过单组分DNA分子的超分子均聚作用实现微粒的自组装

Self-Assembly of Microparticles by Supramolecular Homopolymerization of One Component DNA Molecule.

作者信息

Zeng Jie, Fu Wenhao, Qi Zhenping, Zhu Qiushuang, He Huawei, Huang Chengzhi, Zuo Hua, Mao Chengde

机构信息

Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China.

Biological Science Research Center, Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400716, China.

出版信息

Small. 2019 Jun;15(26):e1805552. doi: 10.1002/smll.201805552. Epub 2019 Feb 8.

DOI:10.1002/smll.201805552

PMID:30734479

Abstract

DNA is a superb molecule for self-assembly of nanostructures. Often many DNA strands are required for the assembly of one DNA nanostructure. For lowering the cost of synthesizing DNA strands and facilitating the assembly process, it is highly desirable to use a minimal number of unique strands for potential technological applications. Herein, a strategy is reported to assemble a series of DNA microparticles (DNAµPs) from one component DNA strand. As a demonstration of the application of the resulting DNAµPs, the design and assembled DNAµPs are modified to carry additional single-stranded tails on their surfaces. The modified DNAµPs can either capture other nucleic acids or display CpG motifs to stimulate immune responses.

摘要

DNA是用于纳米结构自组装的卓越分子。通常，组装一个DNA纳米结构需要许多DNA链。为了降低合成DNA链的成本并促进组装过程，非常希望在潜在的技术应用中使用最少数量的独特链。在此，报道了一种从单一组分DNA链组装一系列DNA微粒（DNAµP）的策略。作为所得DNAµP应用的示例，对设计并组装的DNAµP进行修饰，使其表面带有额外的单链尾巴。修饰后的DNAµP既可以捕获其他核酸，也可以展示CpG基序以刺激免疫反应。