评估用于心脏细胞治疗的干细胞DNA完整性
Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy.
作者信息
Miller Jessica M, Mardhekar Nikhil Maneesh, Rajasekaran Vasanthi, Zhang Jianyi, Kannappan Ram
机构信息
Department of Biomedical Engineering, School of Engineering, School of Medicine, University of Alabama at Birmingham.
Department of Biomedical Engineering, School of Engineering, School of Medicine, University of Alabama at Birmingham;
出版信息
J Vis Exp. 2019 Jan 25(143). doi: 10.3791/58971.
Stem and stem-cell-derived cells have immense potential as a regenerative therapy for various degenerative diseases. DNA is the storehouse of genetic data in all cells, including stem cells, and its integrity is fundamental to its regenerative ability. Stem cells undergo rapid propagation in labs to achieve the necessary numbers for transplantation. Accelerated cell growth leads to the loss of DNA integrity by accumulated metabolites, such as reactive oxygen, carbonyl, and alkylating agents. Transplanting these cells would result in poor engraftment and regeneration of the deteriorating organ. Moreover, transplanting DNA-damaged cells leads to mutations, DNA instability, cellular senescence, and possibly, life-threatening diseases such as cancer. Therefore, there is an immediate need for a quality control method to evaluate the cell's suitability for transplantation. Here, we provide step-by-step protocols for the assessment of the DNA integrity of stem cells prior to cell transplantation.
干细胞及干细胞衍生细胞作为治疗各种退行性疾病的再生疗法具有巨大潜力。DNA是包括干细胞在内的所有细胞中遗传数据的储存库，其完整性对于细胞的再生能力至关重要。干细胞在实验室中快速增殖以达到移植所需数量。加速的细胞生长会导致DNA完整性因活性氧、羰基和烷基化剂等累积代谢物而丧失。移植这些细胞会导致移植效果不佳以及受损器官再生不良。此外，移植DNA受损的细胞会导致突变、DNA不稳定、细胞衰老，并可能引发如癌症等危及生命的疾病。因此，迫切需要一种质量控制方法来评估细胞是否适合移植。在此，我们提供了细胞移植前评估干细胞DNA完整性的详细方案。
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