Departament of Physiology, Federal University of Rio Grande do Sul (UFRGS), Brazil.
Departament of Physiology, Federal University of Rio Grande do Sul (UFRGS), Brazil..
Comp Biochem Physiol A Mol Integr Physiol. 2019 May;231:111-118. doi: 10.1016/j.cbpa.2019.01.028. Epub 2019 Feb 6.
Although widely known for their involvement in the control of carbohydrate and lipid metabolism of vertebrates, the participation of catecholamines (CAs) in the metabolism of invertebrates is less understood. This study was designed to identify the physiological role of Epinephrine (E) in the intermediary metabolism of the burrowing crab Neohelice granulata and how E regulates the metabolism in crabs fed with a high-carbohydrate (HC) or a high-protein (HP) diet. To answer these questions, we evaluated in vivo the effects of E injections on glucose and triglycerides in the hemolymph and tissue glycogen levels of crabs fed with HC or HP diet. An in vitro investigation was carried out to assess the direct effects of E on glycogenolysis, lipolysis and glycolysis pathways in the hepatopancreas, mandibular muscle and anterior and posterior gills of this crab. E injections increased glucose and did not affect triglycerides levels in the hemolymph of either group of crabs, and E decreased glycogen in the hepatopancreas and mandibular muscle only in HP crabs, suggesting that these effects may be mediated by the crustacean hyperglycemic hormone (CHH). When the tissues were incubated with different concentrations of E, the concentration of glucose released to the medium decreased in the hepatopancreas and posterior gills, while glucose oxidation increased in the posterior gills of HP crabs. Incubation with E did not alter any parameter in tissues of HC crabs. These effects suggest that E may be involved in the metabolic response to osmotic stress.
尽管儿茶酚胺（CAs）在脊椎动物的碳水化合物和脂质代谢控制中广为人知，但它们在无脊椎动物代谢中的参与作用却知之甚少。本研究旨在确定肾上腺素（E）在掘足蟹 Neohelice granulata 中间代谢中的生理作用，以及 E 如何调节高碳水化合物（HC）或高蛋白（HP）饮食喂养的螃蟹的代谢。为了回答这些问题，我们评估了 E 注射对 HC 或 HP 饮食喂养的螃蟹血液和组织糖原水平中葡萄糖和甘油三酯的体内影响。进行了体外研究，以评估 E 对这种螃蟹的肝胰腺、下颌肌肉以及前、后鳃中糖原分解、脂肪分解和糖酵解途径的直接影响。E 注射增加了葡萄糖，但未影响两组螃蟹血液中的甘油三酯水平，E 仅在 HP 螃蟹中减少了肝胰腺和下颌肌肉中的糖原，表明这些作用可能是由甲壳动物高血糖激素（CHH）介导的。当组织用不同浓度的 E 孵育时，在肝胰腺和后鳃中释放到培养基中的葡萄糖浓度降低，而在 HP 螃蟹的后鳃中葡萄糖氧化增加。在 HC 螃蟹的组织中孵育 E 不会改变任何参数。这些作用表明，E 可能参与了对渗透胁迫的代谢反应。
