Forage Science, AgResearch, Lincoln Research Centre, Private Bag 4749, Christchurch 8140, New Zealand; Bio-Protection Research Centre, Lincoln, Christchurch, New Zealand.
Forage Science, AgResearch, Lincoln Research Centre, Private Bag 4749, Christchurch 8140, New Zealand.
J Invertebr Pathol. 2019 Mar;162:19-25. doi: 10.1016/j.jip.2019.02.002. Epub 2019 Feb 5.
The application of the biocontrol bacterium Yersinia entomophaga as a foliar spray was assessed for its efficacy against larvae of the diamondback moth, Plutella xylostella. The bacterium was applied as either a broth suspension, or as a biopolymer-based gel foliar spray and compared with commercial insecticides Dipel (Bacillus thuringiensis) and Spinosad. The performance of Y. entomophaga was comparable with that of Dipel. The gel-based formulation extended leaf persistence over that of the basic broth culture spray, while also providing higher initial foliar deposition rates. The bacterium was found to multiply within the P. xylostella larvae to 5.8 × 10 cells per larva, while the median lethal dose (LD) was determined to be 2.69 × 10 cells per larva. Importantly, B. thuringiensis Cry1A-resistant, Cry1C-resistant, indoxacarb/pyrethroid-resistant, and Spinosad-resistant P. xylostella larvae were susceptible to Y. entomophaga.
将生物防治细菌耶尔森氏菌（Yersinia entomophaga）应用于叶面喷雾，评估其对小菜蛾幼虫（Plutella xylostella）的防治效果。该细菌以菌液悬浮液或基于生物聚合物的凝胶叶面喷雾形式应用，并与商业杀虫剂敌百虫（苏云金芽孢杆菌）和 Spinosad 进行比较。耶尔森氏菌的性能与敌百虫相当。基于凝胶的配方延长了叶片的持效期，超过了基本菌液培养喷雾，同时还提供了更高的初始叶面沉积率。发现该细菌在小菜蛾幼虫体内繁殖到每头幼虫 5.8×10 个细胞，而致死中量（LD）被确定为每头幼虫 2.69×10 个细胞。重要的是，对苏云金芽孢杆菌 Cry1A、Cry1C、茚虫威/拟除虫菊酯和 Spinosad 具有抗性的小菜蛾幼虫对耶尔森氏菌敏感。
