卡波西肉瘤相关疱疹病毒（KSHV）的门蛋白 ORF43 对于产生感染性病毒颗粒是必需的。

The KSHV portal protein ORF43 is essential for the production of infectious viral particles.

机构信息

The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat-Gan 5290002, Israel; Department of Chemistry, Bar Ilan University, Ramat-Gan 5290002, Israel; Advanced Materials and Nanotechnology Institute, Bar Ilan University, Ramat-Gan 5290002, Israel.

The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat-Gan 5290002, Israel; Advanced Materials and Nanotechnology Institute, Bar Ilan University, Ramat-Gan 5290002, Israel.

出版信息

Virology. 2019 Mar;529:205-215. doi: 10.1016/j.virol.2019.01.028. Epub 2019 Feb 1.

DOI:10.1016/j.virol.2019.01.028

PMID:30735904

Abstract

Herpesvirus capsid assembly involves cleavage and packaging of the viral genome. The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 43 (orf43) encodes a putative portal protein. The portal complex functions as a gate through which DNA is packaged into the preformed procapsids, and is injected into the cell nucleus upon infection. The amino acid sequence of the portal proteins is conserved among herpesviruses. Here, we generated an antiserum to ORF43 and determined late expression kinetics of ORF43 along with its nuclear localization. We generated a recombinant KSHV mutant, which fails to express ORF43 (BAC16-ORF43-null). Assembled capsids were observed upon lytic induction of this virus; however, the released virions lacked viral DNA and thus could not establish infection. Ectopic expression of ORF43 rescued the ability to produce infectious particles. ORF43 antiserum and the recombinant ORF43-null virus can provide an experimental system for further studies of the portal functions and its interactions.

摘要

疱疹病毒衣壳组装涉及病毒基因组的切割和包装。卡波济肉瘤相关疱疹病毒 (KSHV) 开放阅读框 43(orf43) 编码一个假定的门户蛋白。门户复合物作为一个门，通过该门 DNA 被包装到预先形成的衣壳中，并在感染时注入细胞核。门户蛋白的氨基酸序列在疱疹病毒中是保守的。在这里，我们生成了针对 ORF43 的抗血清，并确定了 ORF43 的晚期表达动力学及其核定位。我们生成了一种不能表达 ORF43 的重组 KSHV 突变体 (BAC16-ORF43-null)。在该病毒的裂解诱导下观察到组装的衣壳;然而，释放的病毒粒子缺乏病毒 DNA，因此不能建立感染。ORF43 的异位表达挽救了产生感染性颗粒的能力。ORF43 抗血清和重组 ORF43 缺失病毒可以为进一步研究门户功能及其相互作用提供实验系统。