State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Virology. 2019 Mar;529:216-225. doi: 10.1016/j.virol.2019.01.029. Epub 2019 Feb 1.
Aquareoviruses contain an 11-segmented double-stranded RNA genome. Previous studies indicated that NS38, a virus-encoded putative single-stranded RNA binding protein, interacts with NS80 in viral inclusion bodies (VIBs). However, the role of NS38 in aquareovirus infection remained unclear. Here, we found that NS38 interacts with inner-capsid proteins (VP1-VP4 and VP6) and the NS80-RNA complex in both transfected and infected cells. Knockdown of NS38 by siRNAs-115/219 clearly reduced viral infection, with decreased mRNA and protein yields. Moreover, NS38 can interact with host cellular eukaryotic translation initiation factor 3 subunit A (eIF3A) in transfected cells, while no association was detected between eIF3A and NS80. This study is the first to define that the NS38 is essential to viral replication. Together, our findings indicate that NS38 might function as a mediator by interacting with viral and host cellular components in VIBs during replication.
水生呼肠孤病毒包含一个 11 个片段的双链 RNA 基因组。先前的研究表明，NS38 是一种病毒编码的假定单链 RNA 结合蛋白，与病毒包涵体（VIBs）中的 NS80 相互作用。然而，NS38 在水生呼肠孤病毒感染中的作用尚不清楚。在这里，我们发现 NS38 在转染和感染细胞中与衣壳蛋白（VP1-VP4 和 VP6）和 NS80-RNA 复合物相互作用。通过 siRNAs-115/219 敲低 NS38 可明显降低病毒感染，mRNA 和蛋白产量降低。此外，NS38 可以在转染细胞中与宿主细胞真核翻译起始因子 3 亚基 A (eIF3A) 相互作用，而在 NS80 与 eIF3A 之间未检测到关联。这项研究首次定义了 NS38 对病毒复制是必需的。总之，我们的研究结果表明，NS38 可能在复制过程中通过与 VIBs 中的病毒和宿主细胞成分相互作用，作为一种介导物发挥作用。
