Castillo B, Smith M A L, Yadava U L
Department of Natural Resources and Environmental Sciences, 1021 Plant Sciences Laboratory, 1201 S Dorner Dr., MC-634, University of Illinois, Urbana, IL 61801-4778, USA Fax-no.: +1-217-244-3469 E-mail:
Department of Plant Sciences, Georgia Agricultural Experiment Stations, Fort Valley State University, Fort Valley, GA 31030-3298, USA, , , , , , GE.
Plant Cell Rep. 1998 Jan;17(3):172-176. doi: 10.1007/s002990050373.
Carica papaya L. (papaya) single somatic embryos (2.0 mm diameter) produced in a high-frequency liquid production system were encapsulated in two different synthetic encapsulation compounds. The frequency of regeneration from encapsulated embryos was significantly affected by (1) the concentration of sodium alginate, (2) the presence or absence of nutrient salts in the capsule, and (3) the duration of exposure to calcium chloride. A 2.5% sodium alginate concentration in a half-strength MS salts base resulted in significantly higher germination frequencies than other treatments. A relatively short (10 min) exposure to CaCl provided uniform encapsulation of embryos and the highest frequencies of successful germination (77.5%). Germinated artificial seeds produced normal plantlets.
在高频液体生产系统中产生的番木瓜（Carica papaya L.）单个体细胞胚（直径2.0毫米）被包埋在两种不同的合成包埋化合物中。包埋胚的再生频率受到以下因素的显著影响：（1）海藻酸钠的浓度；（2）胶囊中营养盐的有无；（3）氯化钙处理的持续时间。在1/2强度的MS盐培养基中，2.5%的海藻酸钠浓度导致的发芽频率显著高于其他处理。相对较短时间（10分钟）的氯化钙处理能使胚得到均匀包埋，并获得最高的成功发芽频率（77.5%）。发芽的人工种子产生了正常的植株。
