Wakhlu A K, Sharma R K
Department of Biosciences, University of Jammu, Jammu-180 006, India, , , , , , IN.
Plant Cell Rep. 1998 Aug;17(11):866-869. doi: 10.1007/s002990050499.
A protocol has been developed for achieving somatic embryogenesis and plant regeneration from petiole-derived callus of Heracleum candicans Wall. Callus was initiated on MS medium supplemented with 0.5 mg l 2,4-D and 0.5 mg l BAP and subcultured on a medium containing double strength MS macrosalts, 1 mg l2,4-D and 0.25 mg l Kn. Numerous globular embryos were formed on the surface of the callus upon transfer to auxin-rich MS medium that lacked cytokinins. The globular embryos differentiated into mature embryos only when 2,4-D was removed from the medium. Mature embryo formation was significantly influenced by the pH of the medium and the addition of AgNO and ABA. Eighty-five percent of the somatic embryos were converted into plantlets when transferred to a medium supplemented with 0.01 mg l BAP and 0.01 mg l IBA. The regenerated plants have been established in soil and appear to be identical to the parent plants in morphology and chromosome number.
已开发出一种从白花独活叶柄愈伤组织实现体细胞胚胎发生和植株再生的方案。愈伤组织在添加了0.5 mg/L 2,4 - D和0.5 mg/L BAP的MS培养基上诱导形成，并在含有双倍强度MS大量元素、1 mg/L 2,4 - D和0.25 mg/L激动素的培养基上继代培养。将愈伤组织转移到缺乏细胞分裂素的富含生长素的MS培养基上后，在其表面形成了大量球形胚。只有当培养基中去除2,4 - D时，球形胚才分化为成熟胚。成熟胚的形成受到培养基pH值以及添加硝酸银和脱落酸的显著影响。当转移到添加了0.01 mg/L BAP和0.01 mg/L IBA的培养基上时，85%的体细胞胚转化为植株。再生植株已在土壤中定植，在形态和染色体数目上似乎与亲本植株相同。
