Ponsamuel J, Huhman D V, Cassidy B G, Post-Beittenmiller D
Plant Biology Division, The Samuel Roberts Noble Foundation, P. O. Box 2180, Ardmore, OK 73402, USA, , , , , , US.
Plant Cell Rep. 1998 Mar;17(5):373-378. doi: 10.1007/s002990050409.
Shoot buds were induced from plumular explants of peanut (Arachis hypogaea L., cv Okrun') preconditioned on medium containing 2,4-dichlorophenoxyacetic acid and kinetin and then transferred to regeneration medium containing benzylaminopurine and β-naphthoxyacetic acid. Buds differentiated 25 days following transfer to regeneration medium. Each explant produced 30 to 40 buds, but only 4 shoots. The remaining buds were dormant and did not produce shoots when maintained on regeneration medium. Shoots were regenerated continuously, however, when explants were subsequently transferred to shoot conversion medium containing 1 µM brassin, benzylaminopurine and β-naphthoxyacetic acid, respectively. Approximately 5 shoots were harvested every 30 days after transfer to shoot conversion medium for up to 7 months. No further shoot production was observed from explants maintained on regeneration medium without brassin. Regenerated shoots could be rooted and produced viable seeds. This procedure provides an efficient and reliable system for regeneration and transformation studies using cv Okrun'.
从花生（落花生L.，品种‘Okrun’）的胚轴外植体诱导出芽，这些外植体先在含有2,4-二氯苯氧乙酸和激动素的培养基上预处理，然后转移到含有苄氨基嘌呤和β-萘氧乙酸的再生培养基上。转移到再生培养基25天后芽开始分化。每个外植体产生30到40个芽，但只有4个芽发育成苗。其余的芽处于休眠状态，当保持在再生培养基上时不会发育成苗。然而，当外植体随后转移到分别含有1 μM油菜素、苄氨基嘌呤和β-萘氧乙酸的芽转化培养基上时，苗会持续再生。转移到芽转化培养基后，每30天大约收获5株苗，持续7个月。在没有油菜素的再生培养基上培养的外植体没有观察到进一步的苗产生。再生的苗可以生根并产生可育种子。该方法为使用‘Okrun’品种进行再生和转化研究提供了一个高效且可靠的系统。
