Chakraborti S P, Vijayan K, Roy B N, Qadri S M H
Central Sericultural Research and Training Institute, Berhampore-742 101, West Bengal, India, , , , , , IN.
Plant Cell Rep. 1998 Jul;17(10):799-803. doi: 10.1007/s002990050486.
A high frequency of tetraploidy was induced in mulberry (Morus alba L.) through apical bud treatment under in vitro conditions. Apical buds from in vitro-grown plants were treated with three different concentrations (0.05, 0.1 and 0.2%) of colchicine in MS medium for 24 h. Tetraploidy at a frequency of 39.4±4.8% was obtained using 0.1% colchicine, whereas the frequency of tetraploidy was significantly reduced to 16.7±2.3% when 0.2% colchicine was used. Morphological, histological and cytological evidence indicated a phenotypic and genomic similarity of in vitro- with ex vitro-induced tetraploids. Rooting of tetraploids was on basal medium containing 2.6 μM NAA. The recovery of tetraploids was 80.8% more efficient using the in vitro method instead of the ex vitro method. The use of the same colchicine medium for up to 4 weeks with additional explants was found to be equally effective for the induction of tetraploidy.
通过体外条件下的顶芽处理，在桑树（桑属白桑种）中诱导出了高频率的四倍体。将离体培养植株的顶芽在添加了三种不同浓度（0.05%、0.1%和0.2%）秋水仙碱的MS培养基中处理24小时。使用0.1%秋水仙碱时获得了频率为39.4±4.8%的四倍体，而使用0.2%秋水仙碱时四倍体频率显著降至16.7±2.3%。形态学、组织学和细胞学证据表明，体外诱导的四倍体与离体诱导的四倍体在表型和基因组上具有相似性。四倍体在含有2.6 μM萘乙酸的基础培养基上生根。与离体方法相比，使用体外方法四倍体的回收率提高了80.8%。发现使用相同的秋水仙碱培养基并添加额外外植体长达4周，对四倍体的诱导同样有效。
