Sreenivasu K, Malik S K, Ananda Kumar P, Sharma R P
National Research Centre for Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India, , , , , , IN.
National Bureau of Plant Genetic Resources, New Delhi-110012, India Fax no.: +91-11-5766420 E-mail:
Plant Cell Rep. 1998 Feb;17(4):294-297. doi: 10.1007/s002990050395.
Efficient plant regeneration via somatic embryogenesis has been developed in pigeonpea. Cotyledon and leaf explants from 10-day-old seedlings produced embryogenic callus and somatic embryos when cultured on Murashige and Skoog (MS) medium supplemented with 10 µM thidiazuron (TDZ). Subsequent withdrawal of TDZ from the induction medium resulted in the maturation and growth of the embryos into plantlets on MS basal medium. The rooted plantlets were transferred and acclimatized on vermiculite where they showed normal morphological characters.
木豆已通过体细胞胚胎发生实现了高效的植株再生。将10日龄幼苗的子叶和叶片外植体接种在添加了10 μM噻苯隆（TDZ）的Murashige和Skoog（MS）培养基上培养时，可产生胚性愈伤组织和体细胞胚。随后从诱导培养基中去除TDZ，可使胚胎成熟并在MS基本培养基上生长成小植株。生根的小植株被转移到蛭石上进行驯化，在那里它们表现出正常的形态特征。
