Chen L Z, Adachi T
Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, Gakuen Kibanadai Nishi 1-1, Miyazaki 889-2192, Japan Fax no.: +81-985-58-2884 E-mail:
Plant Cell Rep. 1998 Apr;17(6-7):508-514. doi: 10.1007/s002990050433.
Somatic hybrids were obtained by polyethylene glycol fusion of cotyledon protoplasts of Lycopersicon esculentum Mill. cv. Kyoryokutoko treated with iodoacetamide (IOA) and suspension-culture-derived protoplasts of L. peruvianum (PI270435) or L. chilense (PI128652). The hybrids were selected by a multiple-step selection procedure relying on the different colors of the fusion partners, IOA treatment of cotyledon protoplasts, and the use of a culture medium which only allowed cotyledon protoplasts to regenerate. The somatic embryos were derived from greenish calli that formed from the fusion mixtures, developed progressively through the globular, heart, and torpedo stages, and finally formed complete plantlets. The excised torpedo-stage embryos could be propagated on a modified medium. The morphology of the somatic hybrids were intermediate to their donor partners, and chromosome observations indicated that the hybrids were tetraploid, hexaploid, and aneuploid.
通过聚乙二醇融合番茄（Lycopersicon esculentum Mill. cv. Kyoryokutoko）经碘乙酰胺（IOA）处理的子叶原生质体与秘鲁番茄（L. peruvianum，PI270435）或智利番茄（L. chilense，PI128652）悬浮培养来源的原生质体，获得了体细胞杂种。通过多步选择程序对杂种进行选择，该程序依赖于融合亲本的不同颜色、子叶原生质体的IOA处理以及使用仅允许子叶原生质体再生的培养基。体细胞胚源自融合混合物形成的淡绿色愈伤组织，逐渐发育通过球形、心形和鱼雷形阶段，最终形成完整的植株。切除的鱼雷形阶段胚可以在改良培养基上增殖。体细胞杂种的形态介于其亲本之间，染色体观察表明杂种为四倍体、六倍体和非整倍体。
