Al-Khaldi Abdullah, Sultan Samar
Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
BMC Endocr Disord. 2019 Feb 8;19(1):19. doi: 10.1186/s12902-019-0350-y.
Sirtuin 1 (SIRT1) and sirtuin 3 (SIRT3) proteins have an important role in counteracting oxidative stress. Although diabetes and hypothyroidism (HT) are both characterized by oxidative stress, the mechanisms are not fully understood. This study investigated the effects of type 1 diabetes (T1D), type 2 diabetes (T2D), and HT on the expression levels of SIRT1, SIRT3, and manganese superoxide dismutase (SOD2).
Gene expression of SIRT1, SIRT3, and SOD2 was measured using real-time PCR. The protein expression of SOD2 and lipid peroxidation (thiobarbituric acid reactive substances) was measured by the TBARS Assay kit and enzyme-linked immunosorbent assay (ELISA) respectively.
The results showed that the SIRT1 and SIRT3 levels were lower in peripheral blood samples from patients with T1D, T2D, or HT than in healthy individuals. Interestingly, the mRNA and protein expression levels of SOD2 were higher in all three patient groups. Lipid peroxidation was higher in the patients with HT than in the healthy individuals.
These results indicate alterations in the expression levels of sirtuins and superoxide dismutase in diabetes and HT, which may be related, at least in part, to the oxidative stress. Identifying such alterations in those patients will pave the way towards the development of drugs to enhance SIRT1 and SIRT3 expression and their activity to prevent the damaging effect of oxidative stress.
沉默调节蛋白1（SIRT1）和沉默调节蛋白3（SIRT3）在对抗氧化应激中发挥重要作用。尽管糖尿病和甲状腺功能减退症（HT）均以氧化应激为特征，但其机制尚未完全明确。本研究调查了1型糖尿病（T1D）、2型糖尿病（T2D）和HT对SIRT1、SIRT3和锰超氧化物歧化酶（SOD2）表达水平的影响。
采用实时聚合酶链反应（PCR）检测SIRT1、SIRT3和SOD2的基因表达。分别通过硫代巴比妥酸反应物质（TBARS）检测试剂盒和酶联免疫吸附测定（ELISA）法检测SOD2的蛋白表达和脂质过氧化水平。
结果显示，T1D、T2D或HT患者外周血样本中SIRT1和SIRT3水平低于健康个体。有趣的是，所有三个患者组中SOD2的mRNA和蛋白表达水平均较高。HT患者的脂质过氧化水平高于健康个体。
这些结果表明糖尿病和HT患者中沉默调节蛋白和超氧化物歧化酶的表达水平发生改变，这可能至少部分与氧化应激有关。确定这些患者中的此类改变将为开发增强SIRT1和SIRT3表达及其活性以预防氧化应激损伤作用的药物铺平道路。
