Kalachev Alexander V, Yurchenko Olga V, Kiselev Konstantin V
A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Palchevsky str. 17, Vladivostok, 690041, Russia.
A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Palchevsky str. 17, Vladivostok, 690041, Russia.
Tissue Cell. 2019 Feb;56:79-82. doi: 10.1016/j.tice.2018.12.004. Epub 2018 Dec 21.
An ultrastructural study of developing spermatids in sea urchins, Strongylocentrotus intermedius, showed that macroautophagy is involved in formation of residual bodies and removal of excessive cytoplasm by spermatids during spermatogenesis in this species. During late stages of spermatogenesis spermatids sequester excessive cytoplasm into vesicles, surrounded by a double membrane. Subsequently, these vesicles fused to one another into larger vacuoles, up to 1.5 μm in diameter. Finally, the vacuoles transformed into residual bodies by condensing their content into finely granular material of varying electron density, separated from cytoplasm by a single membrane. An immunoelectron microscopic study of late spermatids with the antibodies, raised against microtubule-associated protein 1 A/1B-light chain 3 (LC3), which is a marker of autophagosomes, showed that residual bodies in late spermatids of S. intermedius were LC3-positive.
对中间球海胆发育中的精子细胞进行的超微结构研究表明，巨自噬参与了该物种精子发生过程中残余体的形成以及精子细胞对多余细胞质的清除。在精子发生的后期，精子细胞将多余的细胞质隔离到由双层膜包围的小泡中。随后，这些小泡相互融合形成直径达1.5μm的更大液泡。最后，液泡通过将其内容物浓缩成电子密度不同的细颗粒物质而转化为残余体，由单层膜与细胞质分隔开。用针对微管相关蛋白1A/1B轻链3（LC3）（自噬体的标志物）产生的抗体对晚期精子细胞进行免疫电子显微镜研究表明，中间球海胆晚期精子细胞中的残余体是LC3阳性的。
