Aulestia-Viera Patricia Verónica, Gontijo Sávio Morato Lacerda, Gomes Alinne Damásia Martins, Sinisterra Rubén Dario, Rocha Rodney Garcia, Cortés Maria Esperanza, Dos Santos Marinilce Fagundes, Borsatti Maria Aparecida
Department of Stomatology, Faculty of Dentistry, University of São Paulo, Av. Professor Lineu Prestes, 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
Department of Restorative Dentistry, Faculty of Dentistry, Federal University of Minas Gerais, Av. Presidente Antônio Carlos, 6627, Belo Horizonte, MG, Brazil.
Oral Maxillofac Surg. 2019 Mar;23(1):53-61. doi: 10.1007/s10006-019-00747-4. Epub 2019 Feb 8.
Dry socket (DS) is one the most common and symptomatic post-extraction complications; however, no consensus on its treatment has been reached. This study aimed to develop a novel dressing material for DS containing the phenolic agent guaiacol and evaluate its biological properties.
An inclusion complex of guaiacol and β-cyclodextrin (Gu/βcd) was prepared by freeze-drying. Its antibacterial activity over six oral bacteria was analyzed using the microdilution method, and its cytotoxicity in osteoblasts was assessed with the MTT assay. The alveolar healing process induced by Gu/βcd was evaluated histologically after the treatment of DS in rats.
βcd complexation potentiated Gu's antibacterial effect and reduced its cytotoxicity in osteoblasts. Bone trabeculae were formed in the alveolar apices of rats treated with Gu/βcd by day 7. On day 14, woven bone occupied the apical and middle thirds of the sockets; on day 21, the entire alveolus was filled by newly formed bone, which was in a more advanced stage of repair than the positive control (Alvogyl™).
The improvement in Gu's biological properties in vitro and the rapid alveolar repair in comparison with Alvogyl™ in vivo demonstrated the benefits of the Gu/βcd complex as a future alternative for the treatment of DS.
干槽症（DS）是最常见且有症状的拔牙后并发症之一；然而，关于其治疗方法尚未达成共识。本研究旨在开发一种含酚类药物愈创木酚的新型DS敷料材料，并评估其生物学特性。
通过冷冻干燥制备愈创木酚与β-环糊精的包合物（Gu/βcd）。采用微量稀释法分析其对六种口腔细菌的抗菌活性，并用MTT法评估其对成骨细胞的细胞毒性。在大鼠DS治疗后，通过组织学方法评估Gu/βcd诱导的牙槽愈合过程。
β-环糊精络合增强了愈创木酚的抗菌作用，并降低了其对成骨细胞的细胞毒性。到第7天时，用Gu/βcd治疗的大鼠牙槽顶端形成了骨小梁。在第14天时，编织骨占据了牙槽窝的顶端和中间三分之一；在第21天时，整个牙槽被新形成的骨填充，其修复阶段比阳性对照（Alvogyl™）更高级。
愈创木酚体外生物学特性的改善以及与Alvogyl™相比在体内牙槽快速修复，证明了Gu/βcd复合物作为未来DS治疗替代方法的优势。
