Menon Triveni, Nair Sreelaja
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.
Methods Mol Biol. 2019;1920:111-128. doi: 10.1007/978-1-4939-9009-2_8.
Metazoan animals are typically diploid, possessing two sets of a chromosome in the somatic cells of an organism. In naturally diploid species, alteration from the endogenous diploid state is usually embryonic lethal. However, the ability to experimentally manipulate ploidy of animal embryos has fundamental as well as applied biology advantages. In this chapter we describe experimental procedures to convert normally diploid zebrafish embryos into haploid or tetraploid states. We also describe methodologies to verify the ploidy of embryos and the utility of ploidy manipulation in expediting the isolation of mutations using both forward and reverse genetic strategies in zebrafish.
后生动物通常是二倍体，在生物体的体细胞中拥有两套染色体。在天然的二倍体物种中，从内源性二倍体状态的改变通常是胚胎致死的。然而，通过实验操作动物胚胎倍性的能力具有基础生物学和应用生物学优势。在本章中，我们描述了将正常二倍体斑马鱼胚胎转化为单倍体或四倍体状态的实验步骤。我们还描述了验证胚胎倍性的方法，以及在斑马鱼中使用正向和反向遗传策略加速突变分离的倍性操作的实用性。
