Vickaryous Matthew K, Gilbert Emily A B
Department of Biomedical Sciences, University of Guelph, Guelph, ON, Canada.
Department of Surgery, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.
Methods Mol Biol. 2019;1920:219-246. doi: 10.1007/978-1-4939-9009-2_13.
Reptiles (lizards, snakes, turtles, and crocodilians) are becoming increasingly popular as models for developmental investigations. In this review the leopard gecko, Eublepharis macularius, is presented as a reptilian model for embryonic and tissue regeneration studies. We provide details of husbandry and breeding and discuss aspects of embryonic nutrition, egg anatomy, and sex determination. We provide comprehensive protocols for transcardial perfusion, short-term anesthesia using the injectable anesthetic Alfaxan, and full-thickness cutaneous biopsy punches, used in geckos for the study of scar-free wound healing. We also provide modifications to three popular histological techniques (whole-mount histochemistry, immunohistochemistry, and double-label immunofluorescence) and provide details on bromodeoxyuridine (BrdU) labeling and immuno-detection.
爬行动物（蜥蜴、蛇、龟和鳄鱼）作为发育研究的模型正变得越来越受欢迎。在这篇综述中，豹纹守宫（Eublepharis macularius）被作为胚胎和组织再生研究的爬行动物模型进行介绍。我们提供了饲养和繁殖的细节，并讨论了胚胎营养、卵的解剖结构和性别决定等方面。我们提供了用于心脏灌注、使用可注射麻醉剂Alfaxan进行短期麻醉以及用于研究无瘢痕伤口愈合的全层皮肤活检打孔的综合方案。我们还对三种常用的组织学技术（整体组织化学、免疫组织化学和双标记免疫荧光）进行了改进，并提供了溴脱氧尿苷（BrdU）标记和免疫检测的详细信息。
