从非洲爪蟾卵母细胞中分离巴氏小体/种质的方法。
Methods for Isolating the Balbiani Body/Germplasm from Xenopus laevis Oocytes.
作者信息
Butler Amanda, Owens Dawn, King Mary Lou, Aguero Tristan
机构信息
Department of Cell Biology, Miller School of Medicine, University of Miami, Miami, FL, USA.
Department of Developmental Biology, INSIBIO (CONICET-Tucuman National University), Tucuman, Argentina.
出版信息
Methods Mol Biol. 2019;1920:265-275. doi: 10.1007/978-1-4939-9009-2_15.
The Balbiani body (Bb) is a large membrane-less organelle, densely packed with mitochondria, endoplasmic reticulum, proteins, and RNA. The Bb is present in many vertebrate female gametes. In frogs, the Bb is established early during oogenesis and operates as a maternal inherited embryonic determinant that specifies germline identity through the formation of germplasm. We describe here two techniques to isolate the Bb/germplasm from Xenopus laevis primary oocytes.
巴尔比亚尼小体（Bb）是一种大型无膜细胞器，密集分布着线粒体、内质网、蛋白质和RNA。Bb存在于许多脊椎动物的雌性配子中。在青蛙中，Bb在卵子发生早期形成，并作为一种母系遗传的胚胎决定因子，通过种质的形成来确定种系身份。我们在此描述了两种从非洲爪蟾初级卵母细胞中分离Bb/种质的技术。
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